The Bacteriophage Lambda CII Phenotypes for Complementation, Cellular Toxicity and Replication Inhibition Are Suppressed in cII-oop Constructs Expressing the Small RNA OOP.

The Bacteriophage Lambda CII Phenotypes for Complementation, Cellular Toxicity and Replication Inhibition Are Suppressed in cII-oop Constructs Expressing the Small RNA OOP.
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Rajamanickam K, Hayes S,


Rajamanickam K, Hayes S, (click to view)

Rajamanickam K, Hayes S,

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Viruses 2018 03 0710(3) pii E115
Abstract

The temperate bacteriophage lambda (λ) CII protein is a positive regulator of transcription from promoter, a component of the lysogenic response. The expression ofwas examined in vectors devoid of phage transcription-modulating elements. Their removal enabled evaluating if the expression of the small RNA OOP, on its own, could suppress CII activities, including complementing for a lysogenic response, cell toxicity and causing rapid cellular loss of ColE1 plasmids. The results confirm that OOP RNA expression from the genetic elementcan prevent the ability of plasmid-encoded CII to complement for a lysogenic response, suggesting that it serves as a powerful regulatory pivot in λ development. Plasmids with apromoter sequence of 45 nucleotides (45), containing the -10 and -35 regions for, were non-functional; whereas, plasmids with94 prevented CII complementation, CII-dependent plasmid loss and suppressed CII toxicity, suggesting thepromoter has an extended DNA sequence. All three CII activities were eliminated by the deletion of the COOH-terminal 20 amino acids of CII. Host mutations in thelocus, inand ininfluenced CII activities. These studies suggest that the COOH-terminal end of CII likely interacts with the β-subunit of RNA polymerase.

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